四环素诱导的表达 | Tetracycline Inducible Expression
Tet System
Tet Response Element (TRE) 四环素相应元素
Tetracycline-controlled transactivator (tTA) 四环素控制的反式激活因子
Tetracycline operator (tetO) 四环素操纵子
Tetracycline repressor (tetR) 四环素阻遏子
reverse tetracycline-controlled transactivator (rtTA) 反向四环素控制的反式激活因子
tTA-dependent system
In the absence of tetracycline, the tetR portion of tTA will bind these tetO sequences and the activation domain promotes expression.
In the presence of tetracycline, tetracycline binds to tetR. This precludes tTA binding to the tetO sequences and subsequent increase in expression by the activation domain, resulting in reduced gene expression.
不添加四环素，tTA会与tetO结合，上调表达
添加四环素，tTA不与tetO结合，下调表达
rtTA-dependent system.
疑问：这个质粒图的rTetR是用来调控什么基因表达的呢？
